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MIT OCW Biology
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can anyone tell me how to make restriction map? if only fragements size are given which has been digested by different restriction enzymes
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you must have sizes of fragments resulting from the digestion of a DNA sequence by-means each enzyme independently. and then, fragments resulting from digestion with these enzymes at the same time. There are a lot of ideas and some times the experience lead to an incomplete map (when for example 2 fragments can be replaced by each other) as an example, you can see the attached file
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