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What regulates the vacuole in a cell?
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http://www.nature.com/scitable/topicpage/plant-vacuoles-and-the-regulation-of-stomatal-14163334
The nucleus controls all the metabolic activities of the cell, so it is the nucleus that regulates the activities of the vacuoles.
Osmotic concentration inside and outside of the cell.
Sorry, if you mean controls, then the answer is nucleus, if you mean regulate, then the answer is osmosis concentration.
The amount of water or any other substance in and around a cell. the vacuole's purpose is to equalize the pressure from the inside and outside of the cell as well as providing the cell with structure.
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