Question

In this exercise we will look Pesa light absorption in blood. Blood contains red the protein hà | moglobin which transports oxygen around the body. When a steak lgelà | Length of light Pesa Î »= 500 nm is the extinction coefficient of hà | moglobin ÏμH = 21000 cmâ'1 Mâ'1. In the following url is assumed that all mËšalinger phased out ¸ res at 500 nm.

(a) Convert the extinction coefficient of hà | moglobin to the SI unit m2/mol.

(b) The concentration of hà | moglobin in a typical healthy person is cH = 8 10â'3M. Which absorbance A would mËšale Pesa pure blood in a cuvette with vejlà | n

Answer 1

In this exercise we will look Pesa light absorption in blood. Blood contains red the protein hà | moglobin which transports oxygen around the body. When a steak lgelà | Length of light Pesa Î »= 500 nm is the extinction coefficient of hà | moglobin ÏμH = 21000 cmâ'1 Mâ'1. In the following url is assumed that all mËšalinger phased out ¸ res at 500 nm.

(a) Convert the extinction coefficient of hà | moglobin to the SI unit m2/mol.

(b) The concentration of hà | moglobin in a typical healthy person is cH = 8 10â'3M. Which absorbance A would mËšale Pesa pure blood in a cuvette with vejlà | ngden L = 1 cm? (Hint: You resilient outdoor wear ¸ ver not count in SI units
here). Do you think that this weight | RDI of A is realistic to mËšale in a spectrophotometer? (specify)

(c) What is the diluted concentration of hà | moglobin approximately € ™ H, we have in the cuvette (L = 1 cm) to mËšale an absorbance A = 0.5?