in addition to DNA polymerase and primers, the polymerase chain reaction also requires, A. a large amount of DNA, B. restriction enzymes, C. a supply of the four DNA nucleotides, D. complementary sequences of DNA
Let's think about what PCR does. It basically generates lots of copies of a DNA sequence. Now let's see what we've got; DNA polymerase which is an enzyme and primers which basically tell the polymerase where to start creating new DNA. Now let's go through the answers: A. A large amount of DNA - No, why would we need this? The point is to make copies of DNA. B. Restriction enzymes - Nope, restriction enzymes cut DNA. C. A supply of the four DNA nucleotides - There's the answer! You need a supply of the nucleotides in order to build copies of DNA. D. Complementary sequences of DNA - the PCR will generate it's own complement to the DNA template.
Really nice answer!
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