Question

What is a limitation of using electron microscopes to view specimens?

Answer 1

Electron microscopes' disadvantages are :

. They can't operate unless in a complete vacuum
. Local vibrations, radiations and magnetic fields have to be reduced at minimum
. They need very stable and high voltages to work properly (even 5kV)
. Futhermore, they are expensive as hell.

BUT they grant an incredible definition and 3D-wiew...

Answer 2

alright thanks

Answer 3

Additionally the specimen must be killed (if we're talking about cells) and prepared on a slide. You can't view live cells with it.