Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads to which the polynulceotide 5'-TTTTTTTTTTTTTTTT-3' has been covalently attached. After a short incubation, the beads are then extracted from the mixture. When you analyze the cellular nucleic acids that have stuck to the beads, which of the following is most abundant?

a) DNA
b) tRNA
c) rRNA
d) mRNA

Question

Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads to which the polynulceotide 5'-TTTTTTTTTTTTTTTT-3' has been covalently attached. After a short incubation, the beads are then extracted from the mixture. When you analyze the cellular nucleic acids that have stuck to the beads, which of the following is most abundant?

a) DNA
b) tRNA
c) rRNA
d) mRNA

blues · Answer

After transcription, a poly-A tails is added to each nascent mRNA and it is then transported out of the nucleus to be translated. These poly-A tails can base pair with poly-T probes like the ones on the beads.

anonymous · Answer

thanks for your help