Question

How many phosphodiester bonds are created when a DNA fragment is ligated to a plasmid vector using T4 DNA ligase?

Answer 1

If you start with a linearized plasmid and a linear DNA fragment, and they are joined to form a single circle, how many joins have to be made? Each DNA:DNA joining involves creating a phosphodiester bond.

Answer 2

2?

Answer 3

There ya go

Answer 4

Thanks!