A common negative control for standard PCR reaction contains all of the required reagents except:
a) template DNA
b) Mangesium chloride
c) Oligonucleotide primers
d) dNTP's
e) PCR buffer
f) Taq DNA polymerase

Question

A common negative control for standard PCR reaction contains all of the required reagents except:
a) template DNA
b) Mangesium chloride
c) Oligonucleotide primers
d) dNTP's
e) PCR buffer
f) Taq DNA polymerase

anonymous · Answer

Your best bet would be process of elimination...which ones can you eliminate that you /know/ are used in PCR? Taq DNA polymerase would be one; continue on from there.

anonymous · Answer

I tend to always have a control without template to ensure that no contaminants are present in any of my reagents.