Question

While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?

Answer 1

Do you know what amylase does (or can you guess)?

Answer 2

As a hint, try looking up the definition of a substrate in relation to an enzyme, that should get you going in the right direction.