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OpenStudy (anonymous):
Hi, I'm new here. Currently, I'm doing transposon mutagenesis of bacteria. I have tried direct sequencing of transposon flanking region,but it didn't work. I'm trying arbitrary PCR now. However, I'm facing problems of getting bands. Can someone please help me out by giving some suggestions regarding sequencing of the transposon flanking region or arbitrary PCR method? Thank you
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