Hi everyone,

I need your help for some calculations. I need to work out the recipe for making 50ml of 2x sample buffer (Composition of 2x = 20% glycerol, 100mM TrisCl pH 6.8, 4mM EDTA pH 8, 4% (w/v) SDS, 200mM DTT, 0.001% (w/v) bromophenol blue) given that I have been provided with the following stock solutions and salts:

100% glycerol
1M Tris pH 6.8
0.5M EDTA pH 8.0
SDS powder DTT powder (mw=154)
0.25% (w/v) bromophenol blue
sterile water.

Can you please explain to me your workings. Thanks.

Question

Hi everyone,

I need your help for some calculations.  I need to work out the recipe for making 50ml of 2x sample buffer (Composition of 2x = 20% glycerol, 100mM TrisCl pH 6.8, 4mM EDTA pH 8, 4% (w/v) SDS, 200mM DTT, 0.001% (w/v) bromophenol blue) given that I have been provided with the following stock solutions and salts:

100% glycerol
1M Tris pH 6.8
0.5M EDTA pH 8.0
SDS powder DTT powder (mw=154)
0.25% (w/v) bromophenol blue
sterile water.

Can you please explain to me your workings. Thanks.

anonymous · Answer

are you there?

anonymous · Answer

i actually remember doing this very experiment....

kayne · Answer

yes.. it's for a practical actually.. :S

kayne · Answer

I think that I've got it.
Glycerol = 10ml of 100% glycerol
1 M Tris pH 6.8 = 5ml
0.5 EDTA pH 8= 0.4ml
SDS powder = 2g
DDT powder = 1.54g
bromophenol = 0.2ml
and water = 34.4ml