Question

The pUC18 plasmid has a mutation that allows it to produce 500 - 00 copies of the plasmid per cell. What chemical must be added to LB medium (Luria Bertani) to maintain the high copy number plasmid? Explain your answer.

Answer 1

I would say an antibiotic is all you need, the bacterial machinery will interact with the genetic information to create the desired copy number. So as long as natural selection through the addition of an antibiotic is allowed to occur your high copy bacteria will flourish.

Answer 2

oh okay, thank you.