Question

You are tasked with engineering a strain of E. coli that expresses the gene for human epidermal growth factor, EGF. As starting materials, you are given a human cDNA library and a plasmid cloning vector. Your cloning vector contains a MCS (multiple cloning site) which includes EcoRI, BamHI, BglII, NotI, and HindII sequences, the LacZ gene, and the gene for kanamycin resistance. The gene is flanked by EcoRI and HindII sequences. Describe, in detail, how to make E. coli express the EGF gene.