You have completed preparing your buffer and you are ready to go home, but your supervisor informs you just before you leave that you should also prepare 0.5L of 1M Tris-base, 0.5mM EDTA, pH 7.4. The molecular weights are: Tris-base - 121.14, and EDTA -292.25. Show your calculations in preparation of this buffer. The pH of this solution will be basic. In general terms, state how you will pH to 7.4?

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