Which two primers from the list below will allow you to use PCR to amplify this gene? My choices are the first 2.
1. 5'-GTGCA-3'
2. 5'-CACGT-3'
3. 5'-TATGG-3'
4.5'-CCATA-3'
5. 5'-ATACC-3'
6.5'-GGTAT-3'

Question

Which two primers from the list below will allow you to use PCR to amplify this gene? My choices are the first 2. 
1. 5'-GTGCA-3' 
2. 5'-CACGT-3'
3. 5'-TATGG-3' 
4.5'-CCATA-3' 
5. 5'-ATACC-3' 
6.5'-GGTAT-3'

anonymous · Answer

attachment

aaronq · Answer

hmm nope

anonymous · Answer

Ok. I look over what you attached and I am not grasping it. How will those help me to amplify the PCR?

aaronq · Answer

the primers need to be complementary to the 3' end because polymerase elongates in a 5' to 3' direction

aaronq · Answer

so when you said you chose the first 2, one was correct, one wasn't.

anonymous · Answer

Ok so I think that the one that was right was 2. The one on the other side to compliment would have to be 6..GGTAT?

aaronq · Answer

2 is right, 6 is not

anonymous · Answer

Ok. So the one I was able to find was 4 "CCATA" at the end of 3' to 5'. I think I am missing something that I am not getting.