Question

To confirm that the recombinant plasmids obtained were exactly what you wanted, you need to determine the DNA sequence of the PCR products found in several of the recombinant plasmids. To do this, you use the primer 5'-CACG-3'. You carry out a set of four dideoxy sequencing reactions that include template DNA, DNA polymerase, the appropriate buffer, and the radiolabeled DNA primer. In each reaction, you include all four deoxynucleoside triphosphates (dNTPs) and a small amount of one of the four dideoxynucleoside triphosphates (ddNTPs).

Answer 1

determine the DNA sequence of the PCR products, use the primer 5'-CACG-3'.