Excess PCSK9 leads to a decrease in LDL receptor levels. Which one of the following tools would you use in mice to model a potential therapy for humans with familial hypercholesterolemia?
1. RNAi targeting PCSK9 in mice
2. add an extra copy of the PCSK9 gene fused to the GFP coding sequence
3. add an extra copy of the gene encoding PCSK9
4. a microarray comparing mRNA levels in a mouse heterozygous for a loss-of-function PCSK9 allele to the wild-type mouse
5. a SNP chip using

Question

Excess PCSK9 leads to a decrease in LDL receptor levels. Which one of the following tools would you use in mice to model a potential therapy for humans with familial hypercholesterolemia?
1. RNAi targeting PCSK9 in mice
2. add an extra copy of the PCSK9 gene fused to the GFP coding sequence
3. add an extra copy of the gene encoding PCSK9
4. a microarray comparing mRNA levels in a mouse heterozygous for a loss-of-function PCSK9 allele to the wild-type mouse
5. a SNP chip using

aaronq · Answer

it looks like 5. is cut short... regardless, with a microarray you can compare the levels of PCSK9 expressed, so i would say 4.