help!!!!! i ve a project on isolating gentamycin resistant gene from E.fecalis....how can it be isolated from the plasmid Dna ...how to identify the gene....is it by endonuclease restriction ...???

Question

help!!!!!  i ve a project on isolating gentamycin resistant gene from E.fecalis....how can it be isolated from the plasmid Dna ...how to identify the gene....is it by endonuclease restriction ...???

aaronq · Answer

the isolation can be done with endonuclease restriction enzymes once you've identified it's sequence and position. As for the identification, are you given the sequence?