OpenStudy (anonymous):
help please @Ryaan Puromycin is an antibiotic. Its effect is to force the ribosome to detach from the mRNA chain before reaching the stop codon. Explain why this would have a harmful effect on a bacterium.
OpenStudy (anonymous):
@thomaster
OpenStudy (anonymous):
Promycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Puromycin can also be used in some particular conditions for the selection of E. coli transformants. Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain. Animal cells are generally sensitive to concentrations from 1 to 10 µg/ml. (Googled)
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