Question

DNA Restriction enzyme digestion: Original fragment reconstruction (Challenge question ..for some)

Answer 1

Three different restriction enzymes (A,B and C) were used to cut a LINEAR fragment of DNA. Single and double digests were electrophoresed on an agarose gel and the following fragment sizes (in Kb) were observed in the gel:

A: 11, 13 B:6,18 C:3,9,12
A+B=5,6,13 A+C:3,4,8,9 B+C:3,9

Draw a linear DNA fragment with the positions of the restriciton enzyme recognition sites, i.e. draw a restriction map of the molecule that explains these results. Show work by drawing maps of double digests for full points.

Answer 2

Damn it have been to long since I last did a restriction map.

Answer 3

Just to make it a bit more pretty:

Answer 4

Deleted my first drawing I used making the map, but here it is again.