Show how you would make 500 ml of this buffer. I will allow you to use no pH meter, but pretend you were given the following:
1. HEPES dry powder: (CAS Number 7365-45-9, Empirical Formula C8H18N2O4S, Molecular Weight 238.30; pKa 7.5)
HINT: What form of the compound am I giving you?
2. 1.0 M solutions of NaOH and HCl
HINT: You will have to decide to use one of these or both of these, then calculate how much to use.
3. 5.0 M NaCl

Question

Show how you would make 500 ml of this buffer.  I will allow you to use no pH meter, but pretend you were given the following:  
1.	HEPES dry powder:  (CAS Number 7365-45-9, Empirical Formula C8H18N2O4S, Molecular Weight 238.30; pKa 7.5)  
HINT:  What form of the compound am I giving you?
2.	1.0 M solutions of NaOH and HCl 
HINT:  You will have to decide to use one of these or both of these, then calculate how much to use.
3.	5.0 M NaCl