Question

Observation: Bovine pulmonary fibroblasts do not express the protein elastin. TGF-B causes fibroblasts to increase expression of specific genes but not the elastin gene. Apigenin has been shown in other cell types to alter gene expression.

Hypothesis: Bovine pulmonary fibroblasts when treated with both apigenin and TGF-B will express elastin.

Based on the observation, design a controlled experiment to test the hypothesis.

List the conditions required to test the hypothesis include the expected results for each condition. Also include your conclusion.