Question

If i want to check the activity of an enzyme how to go about with it? i would really appreciate if the explanation is from take the enzyme and the substrate..i need to write down each and every step..thank you

Answer 1

what exactly ur looking for @shrutipande9

Answer 2

i have to check the activity of an enzyme...as in the rate at which it converts substrate into product...

Answer 3

wooo then u have to find out the concentration of reactant or concentration of product with time :)

Answer 4

how do u find this rate if u r given just some ug of enzymes and the substrate.

Answer 5

like for eg we take the enzyme beta galatosidase...i can get the conc of reactants. but how to test the products...because we have glcose and galactose.

Answer 6

may BE TLSC or using GC u can find out the concentration

Answer 7

wats tlsc or gc?

Answer 8

ups its called TLC chromatography and Gas chromtography :) may i know what are ur reactants are they organic :)

Answer 9

yep...that would be lactose...because beta galactosidase breaks down lactose into glucose and galactose.

Answer 10

mean u have to find the amount of glucose and galacotose formed after some time then
rate = [product fromed }/ time
those product u can analyse by the HPLC instrument :)

Answer 11

ahhh...ok ok thanks:)