About Special Stain

1) If no endospores are observed on a slide preparation from a culture, does it rule out the organism as an endospore former? (Do you expect to see endospores at all times in a culture?)

2. If a bacterial smear does not show endospores, how will you modify experimental conditions to confirm that the bacteria cannot form endospores and it is not a function of culture conditions?

3. Why do medical microbiologists use additional staining techniques?

Thank you for helping!

Question

About Special Stain 

1) If no endospores are observed on a slide preparation from a culture, does it rule out the organism as an endospore former? (Do you expect to see endospores at all times in a culture?)

2.	If a bacterial smear does not show endospores, how will you modify experimental conditions to confirm that the bacteria cannot form endospores and it is not a function of culture conditions?

3.	Why do medical microbiologists use additional staining techniques? 

Thank you for helping!