Question

Martin is studying sucrase activity. He added sucrose solution + sucrase solution + buffer of pH 5.6 in two tubes. He incubated them at 350C and 1000C respectively. When he performed a confirmatory test, only the first test tube showed enzyme activity. How would you justify the inactivity of the enzyme in the second test tube?

Answer 1

I assume that second tube is under 100 degree celcius incubation. In the second tube case, the temperature is too high for the enzyme to properly functioning and most of cellular activities at this temperature will normally stop because the temperature is exceeding their maximum thermo threshold.