Question

A plasmid DNA sample has a concentration of 200 ng/ul. The sample was prepared as follows prior to lading into a gel for electrophoresis. - 10 uL of sample mixed with 10 uL sterile water - 10 uL of the newly diluted sample mixed with 2 uL of 6X non-denaturing sample buffer (NDSB) - A volume of 6 uL of the NDSB-treated sample was loaded into a well of the gel Assuming no DNA was lost, how many ng of DNA was loaded into the well?

Answer 1

A plasmid DNA sample has a concentration of 200 ng/ul. The sample was prepared as follows prior to lading into a gel for electrophoresis.
- 10 uL of sample mixed with 10 uL sterile water
- 10 uL of the newly diluted sample mixed with 2 uL of 6X non-denaturing sample buffer (NDSB)
- A volume of 6 uL of the NDSB-treated sample was loaded into a well of the gel

Assuming no DNA was lost, how many ng of DNA was loaded into the well?