While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?

Question

shrutipande9 · Answer

hi! welcome to Openstudy!! amylase is an enzyme which breaksdown huge carbohydrate molecules into monosaccharides or disaccharides. every enzyme requires a substrate. if u dnt add a substrate then no reacn can occur.

beccaboo333 · Answer

I'd agree with shrut :)