In the step gradien… - QuestionCove

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OpenStudy (anonymous):

In the step gradient separation, four separate fractions were collected. How were these related to the polarities of the column and of the eluting solvent?

11 years ago

OpenStudy (anonymous):

For this particular lab, the column consisted of C18, a nonpolar substance, while the eluting solvent was 18% Isopropyl alcohol, a polar substance. This is all I have so far........

11 years ago

OpenStudy (anonymous):

@wolfe8

11 years ago

OpenStudy (wolfe8):

@thomaster

11 years ago

OpenStudy (aaronq):

More polar substances associate with the (polar) mobile phase more strongly and elute first, while non-polar substances associate with the stationary phase, are retarded, and elute later. |dw:1392753076392:dw|

11 years ago

OpenStudy (anonymous):

That makes so much sense! Thank you! I'm a visual learner

11 years ago

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