In the step gradient separation, four separate fractions were collected. How were these related to the polarities of the column and of the eluting solvent?

Question

In the step gradient separation, four separate 	fractions were collected. How were these related to the polarities of the column and of the eluting solvent?

anonymous · Answer

For this particular lab, the column consisted of C18, a nonpolar substance, while the eluting solvent was 18% Isopropyl alcohol, a polar substance. This is all I have so far........

anonymous · Answer

@wolfe8

wolfe8 · Answer

@thomaster

aaronq · Answer

More polar substances associate with the (polar) mobile phase more strongly and elute first, while non-polar substances associate with the stationary phase, are retarded, and elute later. |dw:1392753076392:dw|

anonymous · Answer

That makes so much sense! Thank you! I'm a visual learner