Question

You've been provided with fresh juvenile papaya leaves. Your aim is to isolate the protein-coding region of the gene uom and clone it into a plasmid vector for the production of the recombinant protein. You have the complete sequence information for gene uom. For the cloning experiment, what would you use as starting material: genomic DNA or RNA? Justify your answer.

Answer 1

you would wanna use RNA because it contains the "net" sequence, while genomic DNA has introns and exons that need to be spliced out. Then use a reverse transcriptase because RNA is very susceptible to degradation and you will lose the strand very fast.

Answer 2

Okay. thank you and what strategy would you use to clone it with plasmid dna?