Question

Two important steps involved in genetic engineering are cutting the desired gene from its genome, then cutting the vector genome and pasting the gene into it. How is this carried out

Answer 1

Restriction enzymes are used to cleave both the insert and vector DNA at specifc restriction sites. The cleaved insert and vector molecules are mixed and allowed to anneal, forming a hybridized construct. DNA ligase is then added to catalyze the formation of the phosphodiester bonds between the DNA molecules to form the hybrid complex.

Answer 2

This is a highly summarized version of the actual process, which is way too detailed to be placed in this thread.

Answer 3

thanks

Answer 4

You're welcome