Question

the molar absorption coefficient (extinction coefficent) of NADH at 340nm is 6,220l/mol/cm,whereas NAD doesnt absorb at 340nm,

Answer 1

what absorbency will be observed when light at 340nm passes through 1 cm cuvette containing 10 micro meter NADH and 10 micrometer NAD ?

Answer 2

Do you mean microliter, or micromolar?

Answer 3

its mu M

Answer 4

so micromolar, \(\mu M\). So convert micromolar (\(1.0*10^{-6}M\)) to molar, then use the beer lambert law:

\(Abs=\epsilon *c*l\)