i am testing the antibacterial property on ZnO nanoparticles on E.coli ans S.aureus. i have been reading a lot but i couldnt decide which broth to use?
i need to choose between TSA LB and MH. and also i need a significant reason as to why that particular medium is best.

Question

i am testing the antibacterial property on ZnO nanoparticles on E.coli ans S.aureus. i have been reading a lot but i couldnt decide which broth to use?
i need to choose between TSA LB and MH. and also i need a significant reason as to why that particular medium is best.

shrutipande9 · Answer

@thomaster @aaronq

shrutipande9 · Answer

@thomaster could u plz help?

aaronq · Answer

sorry, i dont why one would be better over the other.

nincompoop · Answer

EITHER is okay to use, but the standard growth medium is the Mueller-Hinton agar.  I think the tryptic soy agar is more used for economical purposes.

nincompoop · Answer

But just bear in mind that different bacteria respond differently and sometimes even same species' results may vary.

shrutipande9 · Answer

okay pont noted nin...thanks..:D

nincompoop · Answer

http://www.microbelibrary.org/component/resource/laboratory-test/3189-kirby-bauer-disk-diffusion-susceptibility-test-protocol

aaronq · Answer

i think you should run small pilot experiments with all the broths if you have them available.

shrutipande9 · Answer

thats what i was thinking...gonna check the availibility tomo..thanks both of u..:)