Question

Could somebody help me making a figure a bit more nicely than my handsketch? @ParthKohli

Answer 1

Handsketch attached.

Answer 2

I know my handwriting is epic and not understandable at all :)

Answer 3

@Somy

Answer 4

owh my

Answer 5

you mean making it digitally? or improve your drawing skills :P

Answer 6

Digitally :P

Answer 7

What do you mean??! I got epic drawing skill§!

Answer 8

basically what happens is that:
an R-loop (good ilustration on google), is being cut by a protein called Cas3, inside a huge protein complex called "Cascade".
Next Cascade dissociate, and another single stand is being cut by Cas3. Last step is Cas3 is cutting small DNAs while unwinding double stand DNA.

That is the basic principle, and it is a bit complicated yes, biology knowledge is a huge advantage :)

Answer 9

lol @thomaster
sadly i can't do that @Frostbite

Answer 10

I've been trying my self in illustrator.. the results were catastrophically.

Answer 11

ehh, drawing software?
Even in paint it's easy to draw this.
If you want a professional figure you'd need something better, photoshop-like software.

Answer 12

@thomaster yeah I tried as said adobe illustrator. Do you think you can remake it AND improve the drawing a bit? :)

Answer 13

good luck @thomaster lmao
i would do it if i could

Answer 14

you want it to look like this?
http://www.mdpi.com/ijms/ijms-14-14518/article_deploy/html/images/ijms-14-14518f1-1024.png

(this is probably not the same as what happens in your description but I mean the way they drew things here)

Answer 15

owww this is good

Answer 16

Yeah kinda, just in a little other design way?

Answer 17

Or in fact exactly like that, just with my steps.

Answer 18

edit them then

Answer 19

i don't quite understand your drawing

Answer 20

Is it the last step that is causing trouble?
What happens is that Cas3 is doing exonucleolytic cutting of the DNA in the 3' -> 5' direction while unwinding the duplex. The 5' -> 3' stand is being handled by endonucleolytic cuts, and then 3' -> 5' exonucleolytic cutting of the new stand.

Answer 21

hmmm i'll try doing them, but it'll take a loooooooooooooot of time

Answer 22

when do you need it to be done?

Answer 23

Wednesday the latest :)

Answer 24

lol okay i'll try doing it, by i don't promise that it'll look amazing or so

Answer 25

Any help is very appreciated, I could really use it.

Answer 26

I made this in paint :P

Answer 27

but with some more time I could do way better

Answer 28

good jooob!! @thomaster lol now he can do it in no time

Answer 29

@thomaster Darn paint is better than me! :(
But it looks awesome! Think you could make you version of the full figure?

Answer 30

haha I just made that for fun. I have to work now but I'll give it a show when I'm back.

Answer 31

That would be great, thanks.

Answer 32

whats written in the circle that i made?

Answer 33

In the picture ef.jpeg it is all the same protein Cas3 working on the DNA.

in the 539ee....jpeg it say "crRNA"

Answer 34

The triangle shapes I made to refure to helicase activiy (unwinding of DNA duplex), and the circle ones are nuclease activity.

Answer 35

here you go

Answer 36

Look great! :) Thank you so much

Answer 37

you are welcome :) @Frostbite