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Biology 22 Online
OpenStudy (abhinavb):

I want to check the 5' LTR sequence of my plasmid. I will like to confirm that it is a hybrid 5' LTR and not a wild type HIV-1 5' LTR. Can someone pls guide me on how I can evaluate this?

OpenStudy (aaronq):

How do these differ? Length? -> run a gel Sequence? -> in situ fluorescent hybridization

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