Question

what was used in the 1940s to analyze amino acids that make up proteins?

Answer 1

chro·ma·tog·ra·phy
ˌkrōməˈtägrəfē/
Chemistry
noun: chromatography

the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates.

It would be Chromatography.
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Answer 2

For Future Refrence, when people answer your questions you usually click and give them a medal. :-)