Question 1
In an expression plasmid, the origin of replication: _________________
allows for growth of plasmid containing bacteria in the presence of antibiotics.
Is the position where the base number begins at 1.
is responsible for the replication of mRNA.
allows the bacteria to duplicate the plasmid.

Question 2
Select one answer.
Which DNA sequences must a synthetic gene contain in order for the gene to be inserted into the expression plasmid?
Ribosome binding site.
Origin of replication.
Lac operator.
Restriction enzyme sites.
DNA ligase sites.

Question 3
Select one answer.
Which of these is not used in DNA replication?
RNA polymerase
Helicase
DNA polymerase
Primer

Question 4
Select one answer.
In most bacterial operons, such as the lac operon, which of the following DNA segments is responsible for controlling whether the operon is off or on?

Operator
Repressor
Promoter
lacY gene.

Question 5
Select one answer.
A codon in the middle of an mRNA strand was mutated from UGU to UGA. What type of mutation is this?
frame shift
silent
nonsense
missense

Question 6
Select one answer.
The correct decoding of the following encrypted message: PQRMNO is?
Key(MNO = h PQR = i)
hi
ih

Question 7
Select one answer.
Mitochondria in animal cells contain DNA while only chloroplasts in plants contain DNA.
True
False

Question 8
Select one answer.
Assuming that the genes required for Isoleucine (an amino acid) biosynthesis are contained within an operon. If the cell is grown in the presence of high amounts of isoleucine you would expect to find:
The repressor protein to bind to the operator, allowing transcription.
The repressor protein released from the operator, preventing transcription.
The repressor protein to bind to the operator, preventing transcription.
The repressor protein released from the operator, allowing transcription.

Question 9
Select one answer.
A gene codes for a protein that is 500 amino acids long. Which of the following mutations is likely to be the most deleterious?
a missense mutation at the fifth codon
a missense mutation at the 490th codon
a nonsense mutation at the 490th codon
a nonsense mutation at the fifth codon

Question 10
Select one answer.
The following mRNA: ATG CAT CAT, codes for which of the following amino acids:
Met His His
Val Tyr Tyr
Tyr Tyr Val

Question 11
Select one answer.
Separation of the original DNA strands is due to the action of:
polymerase
helicase
RNA primers
single stranded binding protein

Question 12
Select one answer.
What do UTRs do?
They are regions of the gene that regulate transcription.
They are proteins that regulate transcription.
They are proteins that regulate translation.
They are regions of the gene that regulate translation.

Question 13
Select one answer.
During cell division, the chromosomal DNA becomes
more condensed
less condensed
there is no change in the condensation level.

Question 14
Select one answer.
Which of these is not used in DNA replication?
DNA polymerase
Helicase
RNA polymerase
Primer

Question 15
Select one answer.
The DNA sequence of the expression plasmid adjacent to the ribosome binding site is:
---GGATCCATCTGCGAATTC----
---CCTAGGTAGACGCTTAAG---
It contains a BamH1 site (G^GATCC) and a EcoR1 site (G^AATTC). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end?
EcoR1, followed by BamH1.
EcoR1, followed by EcoR1.
BamH1, followed by BamH1.
BamH1, followed by Eco R1.

Question

Question 1
In an expression plasmid, the origin of replication: _________________
    allows for growth of plasmid containing bacteria in the presence of antibiotics.
    Is the position where the base number begins at 1.
    is responsible for the replication of mRNA.
    allows the bacteria to duplicate the plasmid.

Question 2
Select one answer.
Which DNA sequences must a synthetic gene contain in order for the gene to be inserted into the expression plasmid?
    Ribosome binding site.
    Origin of replication.
    Lac operator.
    Restriction enzyme sites.
    DNA ligase sites.

Question 3
Select one answer.
Which of these is not used in DNA replication?
    RNA polymerase
    Helicase
    DNA polymerase
    Primer

Question 4
Select one answer.
In most bacterial operons, such as the lac operon, which of the following DNA segments is responsible for controlling whether the operon is off or on?

Operator
    Repressor
    Promoter
    lacY gene.

Question 5
Select one answer.
A codon in the middle of an mRNA strand was mutated from UGU to UGA. What type of mutation is this?
    frame shift
    silent
    nonsense
    missense

Question 6
Select one answer.
The correct decoding of the following encrypted message: PQRMNO is?
Key(MNO = h PQR = i)
    hi
    ih

Question 7
Select one answer.
Mitochondria in animal cells contain DNA while only chloroplasts in plants contain DNA.
    True
    False

Question 8
Select one answer.
Assuming that the genes required for Isoleucine (an amino acid) biosynthesis are contained within an operon. If the cell is grown in the presence of high amounts of isoleucine you would expect to find:
    The repressor protein to bind to the operator, allowing transcription.
    The repressor protein released from the operator, preventing transcription.
    The repressor protein to bind to the operator, preventing transcription.
    The repressor protein released from the operator, allowing transcription.

Question 9
Select one answer.
A gene codes for a protein that is 500 amino acids long. Which of the following mutations is likely to be the most deleterious?
    a missense mutation at the fifth codon
    a missense mutation at the 490th codon
    a nonsense mutation at the 490th codon
    a nonsense mutation at the fifth codon

Question 10
Select one answer.
The following mRNA: ATG CAT CAT, codes for which of the following amino acids:
    Met His His
    Val Tyr Tyr
    Tyr Tyr Val

Question 11
Select one answer.
Separation of the original DNA strands is due to the action of:
    polymerase
    helicase
    RNA primers
    single stranded binding protein

Question 12
Select one answer.
What do UTRs do?
    They are regions of the gene that regulate transcription.
    They are proteins that regulate transcription.
    They are proteins that regulate translation.
    They are regions of the gene that regulate translation.

Question 13
Select one answer.
During cell division, the chromosomal DNA becomes
    more condensed
    less condensed
    there is no change in the condensation level.

Question 14
Select one answer.
Which of these is not used in DNA replication?
    DNA polymerase
    Helicase
    RNA polymerase
    Primer

Question 15
Select one answer.
The DNA sequence of the expression plasmid adjacent to the ribosome binding site is:
---GGATCCATCTGCGAATTC----
---CCTAGGTAGACGCTTAAG---
It contains a BamH1 site (G^GATCC) and a EcoR1 site (G^AATTC). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end?
    EcoR1, followed by BamH1.
    EcoR1, followed by EcoR1.
    BamH1, followed by BamH1.
    BamH1, followed by Eco R1.

anonymous · Answer

Question 1
In an expression plasmid, the origin of replication: _________
A)	allows the bacteria to duplicate the plasmid. 

Question 2
Which DNA sequences must a synthetic gene contain in order for the gene to be inserted into the expression plasmid?
A)	Restriction enzyme sites.
Question 3
Which of these is not used in DNA replication?
A)	RNA polymerase
Question 4
In most bacterial operons, such as the lac operon, which of the following DNA segments is responsible for controlling whether the operon is off or on?
A)	Operator
Question 5
A codon in the middle of an mRNA strand was mutated from UGU to UGA. What type of mutation is this? 
A)	nonsense
Question 6
The correct decoding of the following encrypted message: PQRMNO is?
Key(MNO = h PQR = i)
A)	ih
Question 7
Mitochondria in animal cells contain DNA while only chloroplasts in plants contain DNA. (T) or (F) 
A)	False
Question 8
Assuming that the genes required for Isoleucine (an amino acid) biosynthesis are contained within an operon. If the cell is grown in the presence of high amounts of isoleucine you would expect to find:
A)	The repressor protein to bind to the operator, preventing transcription.
Question 9
A gene codes for a protein that is 500 amino acids long. Which of the following mutations is likely to be the most deleterious?
A)	a nonsense mutation at the fifth codon
Question 10
The following mRNA: ATG CAT CAT, codes for which of the following amino acids:
A)	Met His His
Question 11
Separation of the original DNA strands is due to the action of:
A)	helicase
Question 12
What do UTRs do? 
A)	They are regions of the gene that regulate translation. 
Question 13
During cell division, the chromosomal DNA becomes
A)	more condensed
Question 14
Which of these is not used in DNA replication?
A)	RNA polymerase
Question 15
Points: 0 out of 10
The DNA sequence of the expression plasmid adjacent to the ribosome binding site is: 
---GGATCCATCTGCGAATTC----
---CCTAGGTAGACGCTTAAG---
It contains a BamH1 site (G^GATCC) and a EcoR1 site (G^AATTC). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end?
A)	BamH1, followed by Eco R1.

anonymous · Answer

Your welcome next person!