Question

Hello. May I ask have you ever done an Enzyme Lab before? If yes please click on the link below to help me. Thank you!

Answer 1

Yes I have worked in a lab with enzymes, mostly regarding biophysical characterization and kinetics.

Answer 2

Thank yo for your soon reply. But have you done the lab that I need help with?

Answer 3

@Frostbite

Answer 4

Of cause I do not do your work, I may guide you and help you in specific areas in your problem. But I won't make your report.

Answer 5

Yes. I definitely understand that. But do you know the answers of it? And are you sure your answers were correct?

Answer 6

I am sure yes.

Answer 7

And I can also argue for my statements obviously.

Answer 8

I should perhaps say I am educated as a biochemist and protein scientist.

Answer 9

Oh wow...I see. So, can we start yet? Now, I will submit my answers to you to see if I am correct. You just need to correct me? Is is OK? Thanks

Answer 10

Sure.

Answer 11

and I look into it.

Answer 12

Yes. That's more convenient. Thanks. I will choose to send to email. Thanks

Answer 13

Please wait for me 1 min.

Answer 14

Yes. I am just sent it to you. Please check it. Many thanks

Answer 15

In general it all looks correct. But I do got some comments. First of all you write the following for a hypothesis "If the higher concentration of the enzyme will increase the rate of the reaction then the absorbance also will increase"

You talk about absorbance (quantitative measurement), but I do not see those results anywhere. So think fast about, what kind of experiments you are pulling of and what kind of data they produce.
Are the data you produce quantitative or qualitative?

Answer 16

It is trying to answer what the antioxidant does for the human body.

Answer 17

No it got something to do with your hypothesis, to talk about absorbance we need a number if used quantitative.
What I am saying is that you are doing a qualitative experiment: You get out some results to which the answer is: Yes or No.

So your hypothesis isn't to check the absorbance as an indicator of reaction rate.

Answer 18

Sorry...I'm stucked....Can you think of any correct hypothesis?

Answer 19

Sure I can :) not to worry, I send back a revised version. But I just also write here.

Answer 20

I'm very bad at doing a lab, so I always need an expert like you to help me with.

Answer 21

That's so wonderful. my expert. You have save me! ...

Answer 22

And if I make any mistakes, please fix it to me. I'm always willing to study from you and my mistakes too.

Answer 23

Btw: am I under time pressure or can I write relaxed?

Answer 24

You can write with relaxed time, expert. Just to make sure the answers is correct. That's the best. Take your time

Answer 25

The enzyme observation report thingy, I can't open unfortunately, so I gotta trust what you do there is correct, or I can simply explain what I would (with very simple materials that is present in every house hold)

Answer 26

sorry... I will resend it to you? Is it OK?

Answer 27

You can check your mail now

Answer 28

Can you open it?

Answer 29

I'm not sure. It looks like I'm look at the same document as the assignment given.
How about I just look at that last... otherwise I might get confused?

Answer 30

oh Ok...Thanks

Answer 31

Hello,

Oops..I am misunderstood. Yes, I already sent to you the Enzyme Observations Form. Please check it

Answer 32

@KittyT I am back now, and got time to look upon it again. Hope the first corrections was something to work with.

Answer 33

Hello @Frostbite Are you there?

Answer 34

Yes I am here.

Answer 35

@FrostBite Hello. I will redo my work and then I will email you again. So, we can check it together. OK? Thank you for everything

Answer 36

@KittyT Yes I'll be happy to look at the new version ones you've redone it. I hope the corrections are somehow useful. I tried to hold it as scientific as I could, but also as easy as possible to fit your level. :)

Answer 37

@KittyT right here I mean yes.

Answer 38

Ok..Let's start

Answer 39

Okay lets start with the hypothesis.
You wrote about absorbance, but I got to ask: have you at any point in this experiment worked with absorbance?

Answer 40

I don't know. Can you open the instructions of this lab again? I'm suck at this

Answer 41

Well let me ask it like this then: did you use a special kind of machinery for this kind of experiment (cause you need a special machine to obtain absorbance results)?

Answer 42

No it doesn't look like it form the instructions part. Okay so to remake a hypothesis. Our hypothesis is what we expect from the experiment / what do we wish to test.

And I think it would be good say something like:
"Can liver break down hydrogen peroxide and what products are being formed?"

How does that sound to you?

Answer 43

"Can liver break down hydrogen peroxide and what products are being formed?" This will be the hypothesis.. Can a hypothesis be a question? Or it have to start with If...then?

Answer 44

Where I work we don't mind writing it to as a question (cause the hypothesis is OUR thoughts and are to guide us in our research).

Although we can write it as a statement. But we need some more info then. Do you remember the products of when hydrogen peroxide is broken down by catalase?

Answer 45

So basically if you like it to be a statement instead of a question we can :)

Answer 46

Yes, I think I will choose a statement....can you make for me one? please

Answer 47

Well what do you expect that happens? What happens if you add hydrogen peroxide to catalase?

Answer 48

catalase breaks hydrogen peroxide down into oxygen and water; the reaction appears as white bubbles.

Answer 49

There you go. you just made your first hypothesis!!! :)
"Catalase catalyze the reaction of hydrogen peroxide into molecular oxygen and water"

Just a minor rewritten statement of what you said.

Answer 50

What you said there is exactly what a hypothesis should sound like. Well done.

Answer 51

Thank you thank you...just because with your help....so informative. So the hypothesis will be :" Catalase catalyze the reaction of hydrogen peroxide into molecular oxygen and water"

Answer 52

can we add if...then?

Answer 53

Yes. That is a excellent hypothesis. And I shall show you why in 2 sec. Well we can take that in two sec.

Answer 54

Yes..

Answer 55

Okay so with your hypothesis there are two things we gotta check:
1) hydrogen peroxide is made into water and oxygen.
2) catalase only catalyze the reaction.

Now what does it mean that catalase catalyze the reaction?

Answer 56

Or let me ask like this: how does an enzyme work?

Answer 57

Hydrogen-PER-oxide breakdown:
2H2O2 --> 2H2O + O2

Answer 58

When a molecule of the right chemical for that enzyme comes along it will fit exactly into the shape. The area of particular shape is called the active site of the enzyme, as that is where the reaction takes place. The molecule that the enzyme works on is called the substrate.

Answer 59

Yeah not exactly what I meant, but close. [You are not copy pasting from the net are you ;)]

No what I meant is that when an enzyme is speeding up a reaction it is NOT GETTING used during the reaction. This ability we call to catalyze.

Answer 60

oh..okay.

Answer 61

So what about the hypothesis start with "if...then"

Answer 62

Can you make one for me please? I can't think of anything at all. I'm stuck at here...:(

Answer 63

Enzyme Lab:
When you complete the lab, make sure you are answering the questions about the liver. Don’t include the other foods you are testing yet.
You will need to test one food, such as a potato, onion, or apple. This experiment goes on the “Enzyme Observation Form”. This form will contain your hypothesis, data, and conclusions about these additional foods.

Answer 64

So return to what matters:
"2) catalase only catalyze the reaction."

In order to test this, we expect that if we add hydrogen peroxide to the liver a reaction will happened. To show that catalase is a catalyst we will remove a part of the liver to a new glass and add more hydrogen peroxide, IF there is still activity, THEN we know catalase is a catalyst

Answer 65

"if we add hydrogen peroxide to the liver a reaction will happened. To show that catalase is a catalyst we will remove a part of the liver to a new glass and add more hydrogen peroxide, IF there is still activity, THEN we know catalase is a catalyst " So here is the hypothesis right? I think that's good :)

Answer 66

Good good :) (while you wrote I've been sitting writing the report my self. I am willing to share it with you, so long you can promise me it won't be turned it)

Answer 67

I think that the hypothesis is good as well! :)

Answer 68

OMG....I'm crying right now..Because I can say it in my entire life, there's no such good friend and helper like you. Thank you so much and yes I promise I won't turned it in. Please trust me...:) <3

Answer 69

So, you gonna share it using email right?

Answer 70

Ok...so we done with hypothesis part...right?

Answer 71

Yes we are done with the hypothesis I would say yes :)

Answer 72

And I am SUPER GLAD you made the hypothesis your self. Perfect! :D

Answer 73

Thanks...So we move to next questions. Deal?

Answer 74

Can you check for me the part "Purpose of the Lab" too?

Answer 75

Oh yes. I checked that and it is fine for me. But I think you should fast see if hydrogen peroxide is dangerous for the body since you say the liver break down toxic compounds. :)

Answer 76

What do you think? I don't know. Can you check it for me please? Plus, is this lab have to do something with antioxidant? I mean like I have research some of the lab and some they wrote down that:

Purpose of this lab "It is trying to answer what the antioxidant does for the human body."

Hypothesis
In this lab we will test for the importance of antioxidant in the human body.My hypothesis for antioxidant is that it helps fight off free radicals.

Answer 77

Well... they are not exactly showing that. So I would consider it wrong to say.

Answer 78

And I have send a revised version to your email.

Answer 79

Ok....May I ask the report that you have sent it to me. There are some questions at the bottom that you don't fix it, so that's correct right?

Answer 80

Can we take that to the very end? And get all the other stuff done first? :)

Answer 81

Yes...sure

Answer 82

Analysis
Analyze the data you collected in the lab
Characterization of the reaction carried out in the liver:
I suggest you do a table for your self

O2 H2
C - -

E + -

Can you help me with this table. I don't know how to...make this data..

Answer 83

But try read what I wrote. And see if you like it, or you can use it.

Answer 84

It is so informative....I will have a closer look into it. Btw...many thanks. There's no more words so that I can say thank you to you.....:)

Answer 85

You are unsure what I meant with the analyze part?

Answer 86

Yes...I mean like the analyze the data part...

Answer 87

Well. The data analysis is based on 2 experiments:
1) the observation for hydrogen and/or oxygen.
2) the preserved ability to catalyze the reaction.

1) is the one with the table. And the table is based on the stick result. If it pops we know that hydrogen is +. if not we know it is -
same goes for oxygen, if it glows more bright it is +, if not -

Answer 88

Can you do it for me please...i forgot it all.. sorry

Answer 89

Well I have in the document I send you?

Answer 90

Oh oh...I see... Sorry. Nevermind.. Ohh

Answer 91

Hehe no prob :)

Answer 92

Ok..No question. SO informative. Now, I think go to the Notebook Activity Ok? Are u ready??? My awesome, talented expert....<3

Answer 93

The notebook activity on the last page yes?

Answer 94

Yes, exactly...:)