Question

How to find literature value of 1-deoxy-d-xylulose 5-phosphate reductoisomerase kDa

Answer 1

hi I am having trouble find it specifically in e coli if that helps

Answer 2

@Abhisar

Answer 3

Literature value ?

Answer 4

like the value found as the standard for it

Answer 5

its to validate the findings from an experiment, like i got 41-44 kDa for it and I need to compare it to the standard

Answer 6

but I cant find anywhere that it says what the standard is

Answer 7

this paper may help you. Depends of the organism will have different molecular mass, here they compare the sequence of few organism and show the differences. If you know more details you should do a gene bank search about the gene of that enzyme. Let me know if you need more help.

Answer 8

here you got in E coli 43.386 kDa

http://www.ncbi.nlm.nih.gov/protein/EDV66507.1

1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli F11]
GenBank: EDV66507.1
GenPept Identical Proteins Graphics
>gi|190906905|gb|EDV66507.1| 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli F11]
MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVARMVEQCLEFSPRYAVMDDEASAKLLKTMLQ
QQGSRTEVLSGQQAACDMAALEDVDQVMAAIVGAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVK
QSKAQLLPVDSEHNAIFQSLPQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHP
NWSMGRKISVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDMRTPI
AHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTALNAANEITVAAFLAQQ
IRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVVRKEVMRLAS

Theoretical pI/Mw (average) for the user-entered sequence:

10 20 30 40 50 60
MKQLTILGST GSIGCSTLDV VRHNPEHFRV VALVAGKNVA RMVEQCLEFS PRYAVMDDEA

70 80 90 100 110 120
SAKLLKTMLQ QQGSRTEVLS GQQAACDMAA LEDVDQVMAA IVGAAGLLPT LAAIRAGKTI

130 140 150 160 170 180
LLANKESLVT CGRLFMDAVK QSKAQLLPVD SEHNAIFQSL PQPIQHNLGY ADLEQNGVVS

190 200 210 220 230 240
ILLTGSGGPF RETPLRDLAT MTPDQACRHP NWSMGRKISV DSATMMNKGL EYIEARWLFN

250 260 270 280 290 300
ASASQMEVLI HPQSVIHSMV RYQDGSVLAQ LGEPDMRTPI AHTMAWPNRV NSGVKPLDFC

310 320 330 340 350 360
KLSALTFAAP DYDRYPCLKL AMEAFEQGQA ATTALNAANE ITVAAFLAQQ IRFTDIAALN

370 380 390
LSVLEKMDMR EPQCVDDVLS VDANAREVVR KEVMRLAS

Theoretical pI/Mw: 5.67 / 43386.00