You are required to make a solution of Binding Protein A (BPA) half saturated with its binding partner ligand A. The Kd of ligand A for BPA is 1 x 10-5M and it has a molecular weight of 250. How much of ligand A needs to be added to a 2000 ml solution of BPA? Express your answer in mg. as a whole number.

Question

You are required to make a solution of Binding Protein A (BPA)  half saturated with its binding partner ligand A. The Kd of ligand A for BPA is 1 x 10-5M and it has a molecular weight of 250. How much of ligand A needs to be added to  a 2000 ml solution of BPA? Express your answer in mg. as a whole number.

anonymous · Answer

Need help quickly!! :)

aaronq · Answer

first find the concentration with an equilibrium expression, then find it's mass with the volume and the molecular weight.