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OpenStudy (anonymous):
You need to make 50ml of medium 1099. Medium 1099 contains coys solution, 10% fetal bovine serum, 20mM HEPES, 10 micrograms/ml insulin, 1x beta mercapatoethanol and 2mM glutamine. You have the following reagents: coys solution containing 7mM HEPES as well as stocks of 1M HEPES solution, 4mg/ml insulin, 1000x beta-mercapatoethanol and 200mM glutamine. How would you make the medium 1099?
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