You are a scientist using genetic engineering techniques to help people who cannot produce an enzyme needed for proper digestion. How could you use genetic engineering techniques to make transformed bacteria that produce that particular enzyme?

Question

You are a scientist using genetic engineering techniques to help people who cannot produce an enzyme needed for proper digestion.  How could you use genetic engineering techniques to make transformed bacteria that produce that particular enzyme?

love_to_love_you · Answer

@DJPatrickStar

anonymous · Answer

The gene that contains information for the production for that particular enzyme is cut out from a normal person's DNA using special restriction enzymes, which produces 'sticky ends' on the gene. Next, a plasmid (a small molecule of DNA) is removed from a bacteria and mixed with the above gene in the presence of the same restriction enzyme to allow the human gene to fit into the plasmid. Then, heat / electricity is applied to the bacteria to open up the cell membrane so that the plasmid can enter the bacteria. This genetically modified bacteria, now being able to produce that particular enzyme, may be allowed to reproduce in large numbers for the large-scale production of that enzyme for human use.