IF the -pGLO LB/Amp plates had colonies, what two things would you have to change about your lab design and WHY?

-pGLO means no plasmid
LB is a nutrient(constant variable)
Amp-antibiotic

Question

IF the -pGLO LB/Amp plates had colonies, what two things would you have to change about your lab design and WHY?

-pGLO means no plasmid
LB is a nutrient(constant variable)
Amp-antibiotic

anonymous · Answer

@prguan What does the presence of colonies mean? Did you want untransformed bacteria to grow on a plate with AMP in it?

The question is not very clear.  There could be many reasons for this and some of them may not require any real change.

anonymous · Answer

http://www.google.com/url?q=http%3A%2F%2Fsearchingspot.com%2Fwenningbio%2Fbio2site%2Fpgloprotocol.pdf&sa=D&sntz=1&usg=AFQjCNGOrRRDfVV2QGAyGPo62YiDlokf2g