What are the limitations for a scanning electrons microscope ?
The microscope is an powerful magnification tool that can utilize focused beams of electrons to obtain information
You should go read about the differences between scanning electron microscopes and transmission electron microscopes, as well as the requirements for both.
They cant view live organisms
A Scanning Electron Microscope (SEM) is a powerful magnification tool that utilizes focused beams of electrons to obtain information. The high-resolution, three-dimensional images produced by SEMs provide topographical, morphological and compositional information makes them invaluable in a variety of science and industry applications. - See more at: http://www.microscopemaster.com/scanning-electron-microscope.html#sthash.Zecbmrdd.dpuf
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