Question

Use the following structures of amino acids to answer the questions below. Note that the difference in the structures (the side chains) is highlighted by gray shading.

Answer 1

a) A student performed chromatography of the four amino acids and the results were shown in the chromatogram below. If an anion exchange column (column is positively charged) was used in a neutral buffer, assign each amino acid to the corresponding peak in the chromatogram

Answer 2

b) Write 2 – 3 sentences to describe the interactions that will occur for each amino acid with the anion exchange column and its effect on column retention.
c) Write 2 – 3 sentences to describe how the results would differ if a cation exchange column (negatively charged column) were used