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MIT OCW Biology 12 Online
OpenStudy (anonymous):

what dilution should be used for anti GAPDH and anti beta actin in western blotting for H357 cancer cell line lysate??

OpenStudy (anonymous):

it depends on the manufacturer of the antibody. for cell signaling I have used a 1:1000 previously and I will advice you start with that, observe the blot and increase or reduce concentration as appropriate.

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