How to conduct protein expression in bacteria? I searched the internet, and learned that The first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast and simple and usually provides high yields. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, improved genetic tools had made this bacterium more valuable than ever for the expression of complex eukaryotic proteins.

Source: http://www.creative-biolabs.com/bacterial-expression-systems-e-coli-bacillus.html

Question

How to conduct protein expression in bacteria? I searched the internet, and learned that The first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast and simple and usually provides high yields. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, improved genetic tools had made this bacterium more valuable than ever for the expression of complex eukaryotic proteins.

Source: http://www.creative-biolabs.com/bacterial-expression-systems-e-coli-bacillus.html

shadowlegendx · Answer

@Abhisar would be your best bet on this one. Heʻs literally a boss at Biology and such. Iʻm afraid heʻs offline tho :/

candyswift · Answer

Thanks for your advice!

thomaster · Answer

Also it's better to post this in the biology section instead of openstudy feedback :) http://openstudy.com/study#/groups/biology