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OpenStudy (anonymous):

While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?

OpenStudy (anonymous):

Here you have to understand what the function of enzyme (always end up with "-ase") is. To catalyze substrate, the enzymes need something to work on. Then why would it show glucose formation if there is nothing for enzyme to catalyze?

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