Can someone please help Compare and contrast a compound light microscope and a transmission electron microscope. Be sure to discuss the structure and operation of each, as well as the function and usefulness of each when examining specimens
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can someone check my answer @errinss @RandomeQuestions @rayep12 @RiverShinesDragon @ILovePuppiesLol Compound light microscope has a two dimensional image. You can view individual cells and living cells with this microscope. Specimens are placed on glass slides and are seen through light absorption. The compound light microscope is the most used. Transmission electron microscope Has a 2-D view. It has high magnification and a high resolution. Specimens are mounted on thin films of collodion.
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Compound light microscope - Microscope with more than one lens and its own light source. There are ocular lenses in the bonicular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. To ascertain the power of magnification of a compund light microscope, it's needed to take the power of the objective lens and multiply it by the eyepiece which is generally 10x. Although sometimes found as monocular with one ocular lens, the compound binocular microscope is more commonly used today. The first light microscope dates back to 1595, when Zacharias Jansen created a compound microscope that used collapsing tubes and produced magnifications up to 9X. Transmission electron microscope - The transmission electron microscope (TEM) operates on the same basic principles as the light microscope but uses electrons instead of light. What you can see with a light microscope is limited by the wavelength of light. TEMs use electrons as "light source" and their much lower wavelength makes it possible to get a resolution a thousand times better than with a light microscope. The possibility for high magnifications has made the TEM a valuable tool in both medical, biological and materials research.
Compound light microscope: Light illuminated. Two dimensional images. This microscope is the most commonly used. You can view individual cells, even living ones. High magnification, but a low resolution. Specimens are placed on glass slides and are seen through light absorption. Transmission electron microscope: Electron illuminated. Has a 2-D view. Thin slices of specimen are obtained. The electron beams pass through it and seen by means of electron scattering. It has high magnification and high resolution. Specimens are mounted on thin films of collodion or other supporting material on copper grids. Source: http://www.cas.muohio.edu/mbi-ws/microscopes/types.html
As far as usefulness goes, compound microscopes are MUCH less expensive and so can be purchased on most research budgets. They run maybe between USD 200 and 1000. Transmission EM scopes run about $2,000,000 for a decent one. EMs are also more expensive to operate: they require special rooms, and it can be very expensive to produce the electron beam. Of course, the resolution is much higher. Compound microscopes are limited by the wavelength of light (max about 2000x), whereas EM can magnify specimens up to about 500,000x. This is a huge advantage of using EM, especially if you need to resolve tiny objects or structures in your specimen. However, unlike EM, light microscopes can look at live specimens. So, if your work requires the microscopic views of living organisms, the EM is completely useless. Light microscopes also produce color image: the micrographs from EMs are black-and-white. If you need to see color, you can't use EM. Finally, your specimen is destroyed after the lengthy prep and viewing in an electron microscope. The material in light microscopy doesn't have to be destroyed, but the use of special stains may be necessary to see cells or cell structures.
Similarities between all different types of microscope that uses to view the unseen living organism materials and the cells, and differences between all types of microscopes that we can not see both living organism and cell in the all types of microscope, both living organism and cell are seen by different types of microscopes . For example –Compound light microscopes can magnify up to around 1500 times One major advantage of using this type of microscope is that you can view living organisms or living material. To determine the total magnification, of the two lenses multiply the power of the eyepiece by the power of the objective Example, eyepiece lens is 10X (magnifies 10 times) the low power lens is 10X the total magnification is 10 x 10 = 100X or 100 times magnification. And the Electron microscopes are more powerful and much larger than a compound light microscope. Electron microscopes use a beam of electrons and magnetic fields to produce an image of the object, which is then displayed on a computer screen. It is with electron microscopes that scientists can study a cell right down to the atomic and molecular level. Please note, however, because of the way the specimens must be prepared, most electron microscopes can not be used to view living organisms or tissues.
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