Question

What is the best phage display method?

Answer 1

Phage display has become a pivotal tool in immunological and cellular biological research due to its remarkable contribution for the development of new drugs and vaccines. As a kind of the most successful applications, phage display technology has proved its safety and efficiency in the research and development of specific antibodies. Notably, take advantage of phage display platform based on human antibody sequences makes it available to produce fully human antibodies without the ethical and moral issues. In addition, this platform is also a general choice for in vitro protein evolution, a typical example is the well-known antibody affinity maturation. Combine with mutagenesis and phage display technologies, phage display antibody library with size of over 108 (1013 has ever been achieved by Creative Biolabs) can be generated to screen the specific antibodies with high affinity for the development of high efficacy with low side effects antibody drugs. Furthermore, phage display platform is an equally important tool for the development of vaccines and the generation of diagnostic tests for monitoring disease progression and evaluating treatment efficacy. Reference: http://www.creative-biolabs.com/Phage-Display-Platform.html

Answer 2

please close the question if you have been helped thank you :)

Answer 3

Thanks for your replying nillgate. I also found something related on the site you pasted. Antibody libraries are constructed by the genomic information coding for antibody variable domains, which can be derived from B cells of immune or naïve donors. Antibodies are the first proteins which were successfully displayed on the surface of phage by fusing the coding sequence of scFv or Fab to the coat protein. Due to the smaller size, scFv libraries are genetically more stable than Fab libraries, while Fab libraries lack the tendency to form higher molecular weight species, such as dimers and trimers, which can simplify the selection and characterization. Through binding the specific target, the interest antibody can then be eluted and retrieved. http://www.creative-biolabs.com/phage-display-service.html