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Biology 14 Online
OpenStudy (afro):

GUYS which is it? is it the Rna primer that brings in the triphosphate group????? which Dna polymerase 3 comes back into cut off that 2 and make it one phosphate group in Dna replication??

OpenStudy (afro):

Kira_Yamato

OpenStudy (afro):

caozeyuan

OpenStudy (caozeyuan):

I kind of didnt understand your question

OpenStudy (afro):

in the process of DNA replication, the Rna primer is put during the process of the triphosphate placement right?

OpenStudy (caozeyuan):

next time do @ before names, like @Afro

OpenStudy (afro):

oh ok

OpenStudy (afro):

with the RNA primer putting the complementary nucleotide bases, it brings the triphosphate group right where DNA polymerase 3 comes back and slices it off?

OpenStudy (caozeyuan):

"process of the triphosphate placement" I dont understand this term becuase I've been studying biology for 6 years and never heard of it

OpenStudy (afro):

in Dna replication there is a placement of 3 phosphates onto the nucleotides on the replication forks

OpenStudy (caozeyuan):

sorry but Im not recalling anything, maybe Im too rusty

OpenStudy (afro):

is that how complicated biochem is jeezus

OpenStudy (afro):

you forget lol

OpenStudy (afro):

im gonna have to major in it jeeezus, for fornesic science

OpenStudy (caozeyuan):

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OpenStudy (caozeyuan):

it is as much as I can recall about replication initiation

OpenStudy (afro):

nooo i know this i'm saying which Polymerase brings in the triphosphate group

OpenStudy (afro):

awwwwwwwwww mannnn

OpenStudy (caozeyuan):

so which polymerase actually synthesis new DNA, that DNA Pol III isnt it

OpenStudy (caozeyuan):

let me double check

OpenStudy (afro):

ya which one brings in the triphosphate group is it the RNA primer or Dna pol 1?

OpenStudy (caozeyuan):

its definitely not primase, and the RNA primer doesnt do anything

OpenStudy (caozeyuan):

but I am not sure if it is Pol III or pol I

OpenStudy (afro):

so it's not dna POlymerase pol 1, i know for sure Rna primer comes firs tso the DNA polymerase can create the strand from there

OpenStudy (afro):

but i don't know which enzyme slices off the 2 phosphate groups and leaves the 1, and i'm confused as to which one brings the 3 phosphate group

OpenStudy (caozeyuan):

yes, so Primase is what meks the primer for synthesis, and Pol III is the enzyme doing the actual replicaiton

OpenStudy (caozeyuan):

I think its Pol III

OpenStudy (afro):

so wait POL 3 comes back around and cuts off the triphosphate?

OpenStudy (afro):

so RNA primer brings the triphosphate

OpenStudy (caozeyuan):

it does things in one go, it doesnt "come back"

OpenStudy (afro):

and DNA pol I is the merase that attaches it's strand to the rna primer and replaces the bases

OpenStudy (caozeyuan):

RNA primer DOES NOTHING

OpenStudy (caozeyuan):

I think most of job is done by Pol III with some help from Pol I and Pol II

OpenStudy (afro):

WAIT WHAT, STOP IT STOP IT, RNA PRIMER CREATES the existing STRAND so DNA POLYMERASE one can GO from there, DNA polymerase can't work off anything

OpenStudy (caozeyuan):

Primase synthesis a primer and make it attach to DNA

OpenStudy (afro):

it has to have a existing strand before it can replicate

OpenStudy (afro):

wait what im so confusedddddd

OpenStudy (caozeyuan):

calm down

OpenStudy (caozeyuan):

primer is made by primase and the primer attaches to the DNA so that Pol III have somethng to extant

OpenStudy (afro):

so lemme get this straight the Primer is made by DNA 1 polymerase which attaches the triphosphate group?

OpenStudy (caozeyuan):

NO

OpenStudy (caozeyuan):

PRIMASE makes primer

OpenStudy (afro):

which PRIMASE???

OpenStudy (caozeyuan):

DNA Primase, there is no Primase 1 or 2 or 3

OpenStudy (caozeyuan):

just a single primase

OpenStudy (afro):

DNA primase ??????

OpenStudy (afro):

so which one brings in the triphosphate group??????, and then slices it off to make it attach to the sugar nitrogenous base?

OpenStudy (caozeyuan):

you mean the triphosphate on the new nuclotide?

OpenStudy (afro):

yessssssss, which enzyme brings that in

OpenStudy (caozeyuan):

DNA Polymerase III

OpenStudy (afro):

S0 which one slices off the triphosphate??? poly 3?

OpenStudy (caozeyuan):

yes

OpenStudy (afro):

so THIS enyzme does the whole process of attaching it self and slicing it off

OpenStudy (afro):

and so let me get this straight RNA primer isn't thte beginning of a strand where as DNA poly comes in and adds its complementary bases replacing that primer

OpenStudy (caozeyuan):

you are talking another story down the road so let me try clear things up

OpenStudy (caozeyuan):

first step RNA primer is synthesised by primase and is attached to the ssDNA template

OpenStudy (afro):

so it gets synthesised by DNA primase, THEN it attaches to the strand thus making the strand t

OpenStudy (afro):

then DNA poly comes in and attaches, then begins to replace it and bring in the triphosphate?

OpenStudy (caozeyuan):

then Pol III comes in and make the new DNA strand. At this time, the primer just sits there and nobody really care about it.

OpenStudy (caozeyuan):

replace primer?

OpenStudy (afro):

yea place the bases with DNA bases

OpenStudy (afro):

replace

OpenStudy (caozeyuan):

you dont replace primer until you are done replicating

OpenStudy (afro):

wait ohO_O? so it's just there

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